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#1. Lectin staining of cultured CNS microglia - PubMed
由 CA Colton 著作 · 1992 · 被引用 80 次 — Staining was specific for microglia in the mixed glial cultures and was dose dependent. In addition, microglial lectin binding could be reduced or blocked ...
#2. An optimized protocol for combined fluorescent lectin ...
Lectin /antibody staining with fluorescently tagged lectins or antibodies should be ideally performed in frozen (cryostat) tissue sections ...
#3. Imaging with Lectin Fluorescent Conjugates - Thermo Fisher
Fixed and permeabilized osteosarcoma cells simultaneously stained with the fluorescent lectins Alexa Fluor 488 concanavalin A (Con A) (Cat. No.
#4. Lectin staining of cultured CNS microglia. - SAGE Journals
To provide more information on the interaction of various lectins with microglia, we have exam- ined lectin staining pattems in isolated cultured microglia and ...
#5. Lectin staining and flow cytometry reveals female-induced ...
Lectin staining and flow cytometry reveals female-induced sperm acrosome reaction and surface carbohydrate reorganization.
Lectins are carbohydrate-binding proteins that are highly specific for sugar groups that are part of other molecules, so cause agglutination of particular ...
#7. CF® Dye PNA Lectin (Arachis hypogaea) - Biotium
Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and ...
#8. Lectin binding to formalin-fixed paraffin sections
isolated by a protein A-Sepharose 4B column. (Pharmacia) either for use in an indirect antibody staining method, or after conjugation to peroxidase8 were used ...
#9. Lectin Staining of Microvascular Glycocalyx in Microfluidic ...
Extravasation of MDA-MB-231 breast cancer cells from perfusable endothelial lumens was observed with confocal imaging of lectin-stained ...
#10. Histochemical Staining Using Lectin Probes - Springer Nature ...
In histochemistry and cytochemistry, lectins are often used as probes for the localization of carbohydrates in cells and tissues. With lectins, cells and ...
#11. Histological techniques 5. Lectins. Atlas of plant and animal ...
Lectins are proteins used to recognize and bind to different types of carbohydrates and carbohydrate moieties. ... Lectin staining Figure 1.
#12. Lectin Staining Kits - EY Laboratories, Inc.
Kit Contents. Kit #1: These kits contain 1mg each of the following unconjugated or labeled lectins: Con A, DBA, SBA, WGA, UEA- ...
#13. Lectin staining —Bio-101
Each lectin was diluted to 1:25 and incubated with fixed embryos overnight ... evaluated by eye for enriched staining on macrophages compared to other tissues.
#14. Brasil - Lectin staining patterns in human gastric mucosae with ...
Lectin staining patterns in human gastric mucosae with and without exposure to Helicobacter pylori. Padrões de ligação de lectinas em mucosa gástrica humana ...
#15. Rapid lectin-based staining procedure for the detection of the ...
The staining procedure involves an initial incubation of a fixed kidney ... evaluations but the lectin stain was able to detect parasites In ...
#16. Sambucus Nigra Lectin (SNA, EBL), Fluorescein labeled
Fluorescein labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
#17. Lectin RCA-I specifically binds to metastasis-associated cell ...
Candidate lectins were then verified by lectin-based flow cytometry and immunofluorescent staining assays using both TNBC/non-TNBC cancer ...
#18. Lectin-mediated staining followed by confocal imaging. Cells ...
4). We next performed lectin staining of the cells followed by confocal assessment. In both normal and cancer cells, FITC-conjugated WGA and SNA lectins showed ...
#19. Endogenous lectins from cultured cells: Nuclear localization of ...
higher levels of the lectin carbohydrate-binding protein 35 ... lost their nuclear staining and underwent a general decrease in.
#20. DEVELOPMENTAL STAGE-SPECIFIC CHANGES IN LECTIN ...
Fluorescence staining of embryonic day 13 (E13) cerebellar cells with FITC ... As a positive control for FITC lectin staining of disso-.
#21. Health & Environmental Research Online (HERO) | US EPA
Lectins are sensitive probes which bind carbohydrate structures specifically. In this study, we modified the lectin staining procedure for ...
#22. Immunohistochemical and lectin.pdf - Digitum
principal cells, the lectin staining was observed in the ... Immunohistochemistry and lectin histochemistry of the alpaca efferent ducts. Table 1.
#23. A Recombinant Fungal Lectin for Labeling Truncated Glycans ...
To examine the capacity of rPVL to recognize cells derived from human cancer tissues, lectin staining of several cell lines was performed ...
#24. Lectin from Bandeiraea simplicifolia (Griffonia ... - Sigma-Aldrich
Application. Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection in murine astrocytes culture by staining the ...
#25. Lectin Histochemistry Shows WGA, PHA-L and HPA Binding ...
The aim of the present study was to use lectins, carbohydrate-binding ... Negative controls and the inhibition of lectin staining with hapten sugars were ...
#26. Lectin histochemistry of microvascular endothelium in chick ...
Key words Muscle capillaries · Lectin staining · Avian muscles · Chick/quail chimera. Introduction. The evaluation of the capillary density in skeletal mus-.
#27. Identification of nucleated cells in urine using lectin staining
We now report a method to differentiate urinary cell types using lectin staining. Twenty-five lectins were examined with respect to their binding pattern on ...
#28. Lectin Staining Kit - GlyCORE Imaging Core
Products, RRID, Store Location, Components. Lectin Kit I, AB_2336252, 4 degree Fridge (3rd Floor), Concanavalin A. Glycine max (soybean) agglutinin.
#29. Visualization of Mouse Choroidal and Retinal Vasculature ...
Tomato lectin (TL; Lycopersicon esculentum agglutinin) recognizes ... For GSL-IB4 or TL staining on cryosections, fluorescently conjugated lectins were ...
#30. Revealing biomedically relevant cell and lectin type ...
This theme of mutually specific protein(lectin)–glycan ... Glycoclusters as inhibitors of tissue staining by lectins: 1. light microscopy.
#31. Effects of different fixatives on staining patterns of four lectins ...
Fixatives have significant influence on lectin histochemistry of tissue sections; however, their roles in lectin fluorescent staining of ...
#32. Evaluation of a Fluorescent Lectin-Based Staining Technique ...
Evaluation of a Fluorescent Lectin-Based Staining Technique for Some Acidophilic Mining Bacteria. Authors: Dee Jay Fife, Debby F. Bruhn, ...
#33. Flow cytometric analysis of lectin-stained haemocytes of the ...
by flow cytometric analysis with and without staining by 3 lectins: soybean agglutinin (SBA), ... honeybee / haemocytes / flow cytometry / lectin staining.
#34. Erithryna cristagalli (EC lectin) staining: - Johns Hopkins ...
Erithryna cristagalli (EC lectin) staining: 1- Bake the slides 2hs to ON @ 40°C. 2- Wash 2x 10 min in TBS (50 mM Tris-HCl pH=7,6; 150mM NaCl).
#35. Absence of the Lectin Activation Pathway of Complement ...
Complement activation via the lectin pathway (LP) is initiated by the ... Staining was developed using diaminobenzidine (DAB) substrate ...
#36. Carbohydrate Binding Specificity of a Fucose-specific Lectin ...
Aleuria aurantia lectin (AAL) and Lens culimaris agglutinin-A (LCA) have been ... Cytochemical Staining with Lectins—MEF cell cultures were ...
#37. Lectins Application and Resource Guide - 2BScientific
Tonsil secfion (FFPE): Stained using CY®3 conjugated SNA lecfin (red-orange). Mounted in VECTASHIELD. HardSet Anfifade Mounfing Medium with DAPI (blue). Page 3 ...
#38. Triple lectin staining of trichostrongyle eggs from naturally ...
Triple lectin staining of trichostrongyle eggs from naturally infected small ruminants. Author: Ibrahim Abbas, Yara M. Al-Kappany, Louise M.N. Monga, ...
#39. Histochemical Evaluation of Human Prostatic Tissues with ...
These lectins also showed staining differences between BPH and PCa; the latter showing decreased staining intensity with an increased degree of malignancy. PNA ...
#40. Application of fluorescently labelled lectins for the study of ...
Fluorescently labelled lectins have been widely used to stain microbial extracellular glycoconjugates in natural and artificial environments, and to study ...
#41. CF®488A Dye PNA Lectin (Arachis hypogaea) - CliniSciences
PNA lectin is a widely used histological fluorescent stain and is labeled with our superior CF® dyes. It is specific for terminal β-galactose and binds ...
#42. Lectin histochemical analysis of uterine natural killer cells in ...
However, the more recently developed dolichos biflorus agglutinin (DBA) lectin staining was demonstrated to be a more sensitive method for ...
#43. Lectin histochemistry in the developing
The binding sites of several lectins (Con-A, SBA, WGA, PNA, RCA-I, UEA-I, DBA) were studied in the ... Prior to PNA, RCA and DBA lectin staining, some.
#44. Lectin staining | Anemone Lab at Newcastle University
Tag Archives: Lectin staining ... Lectins are glycan-binding proteins that are highly specific for glycan-conjugates and can be used to ...
#45. Glycoconjugate Profiles of Insect Spermatogenesis: Lectin ...
1 June 1997 Glycoconjugate Profiles of Insect Spermatogenesis: Lectin Cytochemical Analysis of ... Lectin staining pattern in cricket testicular germ cells.
#46. Immunohistochemistry protocol for Ulex Europaeus Lectin 1 ...
Anti Ulex Europaeus-1 lectin antibody (NB110-13922) ... In case of excessive background staining remove aggregates from antisera by centrifuging for 15 ...
#47. Lectin Gold Staining Kit General Information
Lectin Gold Staining Kit. General Information. (Cat. No.: LGS-01). INTENDED USE. This kit is designed for light and electron microscopic study of ...
#48. Synthesis and Evaluation of Protein–Phenylboronic Acid ...
Glycan-binding molecules, such as lectins, are very important tools for ... S. H. Cell type-specific lectin staining of the tracheobronchial ...
#49. Assessment of lectin-binding analysis for in situ detection of ...
The biofilms were stained with various fluor-conjugated lectins and three-dimensionally examined by confocal laser scanning microscopy (CLSM). The staining ...
#50. Lectin staining and glycan analysis of podocalyxin (PC) in ...
(A) Kidney sections stained with PNA, RCA-I, LTA, and DBA lectins (from left panels to right panels, respectively) in 3-month-old ht (upper ...
#51. Lectins - Pathology Outlines
Stains & molecular markers - Lectins. ... lectin receptors; Ulex europeaus lectin combines with H antigen that corresponds to O blood group.
#52. Biofilms - 第 149 頁 - Google 圖書結果
Multiple Staining With the standard CLSM equipped with an Ar–Kr laser (lines 488 nm, ... 2A, see color insert), (4) nucleic acid stain lectin lectin (Figs.
#53. Detection of bacterial biofilms using covalent lectin binders
Biofilm of a clinical isolate of Pseudomonas aeruginosa stained with fluorescent dyes (green: living cells; red: dead cells).
#54. Carbohydrate-binding Proteins (Anti-Glyco Antibodies and ...
Lectin has long been well-known as tools for detection and analysis of functional ... cell-staining, inhibition assay for cell adhesion, ...
#55. Proceedings of the Fifth Lectin Meeting Bern, May 31–June 5, ...
Fluorescent-lectin staining and analysis. Fluorescein-conjugated lectins were used at sub-agglutinating concentrations to stain the different populations of ...
#56. Protides of the Biological Fluids: Proceedings of the ...
a) b) Since the lectin staining experiments described here were performed on 2-3 ... or that lectins would bind to some extracellular material that would be ...
#57. Cumulated Index Medicus
... Further studies of lectin binding by villous and extravillous Mosaic lecun and enzyme staining patterns in rat skeletal trigeminal nuclei in the ral .
#58. Immunoglobulins—Advances in Research and Application: 2012 ...
(2012 JAN 3) Indiana University School of Medicine, Gary: Application of WGA Lectin Staining for Visualization of the Connective Tissue in Skeletal Muscle, ...
#59. Glycosciences: Status & Perspectives - 第 577 頁 - Google 圖書結果
(1991) Radiation dose-related changes in lectin-staining pattern in oral squamous cell carcinoma. In Br. J. Rad. 64:124. Boyd W (1970): A textbook of ...
#60. Glycan Detection - Lectenz® Bio
Immunohistochemistry is a technique that uses binding agents, such as antibodies or lectins, to detect and stain antigens in a tissue section. While the general ...
#61. Immunohistochemistry - SCIENTIST CINDY
Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) Lectins are proteins that bind to the oligosaccharide chains on glycoproteins on the plasma ...
#62. The Protein Protocols Handbook - 第 847 頁 - Google 圖書結果
Make sure that the lectin solution always covers the membrane surface during incubation. In contrast to the manufacturer's instructions the staining ...
#63. The niacin receptor HCAR2 modulates microglial response ...
... plaques [C-type lectin domain containing 7a (Clec7a) + ] compared with ... (A) IHC staining of amyloid plaques with thioflavin S (Thio-S; ...
#64. Yeast Lectin (Concanavalin A) protocol | Cherry Biotech
The following protocol explains how to use the temperarture controller CherryTemp when yeast cells are mounted on lectin coated slides.
#65. Omentin-1/ITLN1 in inflammatory conditions | JIR - Dove ...
A double staining using omentin-1/ITLN1 antibody and anti PAX8 ... is a calcium-dependent galactose-binding lectin and its homologue was ...
#66. HHS Public Access - CORE
stained with anti-β-1-chicken integrin (Sigma Chemical Co., ... immunoglobulins, proteoglycans, and lectins (42), which are not found on artificial.
#67. zélia silva - Pos Doc Researcher - Faculdade Ciências e ...
Halilectin-3, a lectin from the marine sponge Haliclona caerulea, induces apoptosis and autophagy in human breast cancer MCF7 cells through caspase-9 ...
#68. Hypocomplementemia in primary Sjogren's syndrome - Paperity
Evans et al. reported the presence of positive staining for C9 around the ... the mannan-binding lectin pathway of complement activation in patients with ...
#69. Immunology - Mannose-Binding Lectin & C-Reactive Protein
#70. What Are String Beans Good For | LastDropMugs
Yet, if you eat them, lectins are resistant to digestive enzymes. ... why does nigrosin not stain bacterial cells/structures? nigrosin is negatively charged ...
#71. LECTIN CONTROL FORMULA - Klaire Labs
LECTIN CONTROL FORMULA. Unique blend supports against problematic lectins for optimal digestive health †. 120 CAPSULES. SKU. CP0201.
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