Reporter gene | Reporter genes and their importance | Reporter gene assay | What are reporter genes? ... <看更多>
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Reporter gene | Reporter genes and their importance | Reporter gene assay | What are reporter genes? ... <看更多>
#1. Reporter gene assay - 盟基生物科技股份有限公司
一、產品介紹. 基因表達在細胞內很難適時被偵測,為了避開這些限制與困難,研究者於有興趣的基因後面設計了「報導基因(reporter gene)」,報導基因通常為一種可以被偵 ...
#2. Reporter Assays - Promega Corporation
Reporter assays provide a way to easily measure a biological response. There are many reporter genes available such as alkaline phosphatase, β-galactosidase, ...
#3. Reporter Gene Assays | Thermo Fisher Scientific - TW
Reporter gene assays are typically used to measure the regulatory ability of an unknown DNA-sequence. This is done by linking the unknown promoter sequence to ...
#4. Introduction to Reporter Gene Assays - YouTube
Learn more about the basic concept of reporter assays using luciferase, and how a dual- luciferase assay works. To learn more about this ...
#5. Reporter genes and their importance - YouTube
Reporter gene | Reporter genes and their importance | Reporter gene assay | What are reporter genes?
In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in ...
#7. Reporter gene assays - Berthold Technologies GmbH & ...
The main purpose of the reporter gene assay is to investigate the promoter of a gene of interest, i.e. the regulation of its expression.
#8. Reporter gene assays - PubMed
Reporter gene assays are versatile and sensitive methods of assaying numerous targets in high-throughput drug-screening programs. A variety of reporter ...
#9. 產品介紹--Luciferase Reporter Assay Kits - 伯森生技
Luciferase Reporter Assay Kits. 美商PerkinElmer 公司針對各種偵測靈敏度與訊號維持時間要求,研發設計出多款Luciferase 報導基因偵測產品,以滿足各類型實驗室需求 ...
#10. Vazyme產品專區| 鼎捷生技有限公司
Dual Luciferase Reporter Assay Kit可靈敏高效地檢測受基因元件調控的Luciferase表達, 通常將轉錄調控元件克隆於Firefly luciferase的上游,或把3'UTR調控區克隆 ...
#11. Reporter Gene Assays - PerkinElmer
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent ...
#12. TOOLShinning Dual Luciferase Reporter Assay Kit|圖爾思生技
TOOLShinning Daual Luciferase Reporter Assay Kit detects gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence ...
#13. Luciferase Assay - an overview | ScienceDirect Topics
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene. Unlike the ChIP or EMSA assays, ...
#14. The Luciferase Reporter Assay: How it Works - Bitesize Bio
The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, ...
#15. Reporter gene comparison demonstrates interference of ...
Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter ...
#16. A Deep Dive Into the Luciferase Assay: What It is ... - GoldBio
A luciferase reporter assay is a test that investigates whether a protein can activate or repress the expression of a target gene using luciferase as a reporter ...
#17. Cignal Reporter Assay Kits - QIAGEN
Cignal Reporter Assays provided in the Cignal Reporter Assay Kit enable rapid, sensitive, and quantitative assessment of signal transduction pathway ...
#18. SpectraMax Glo Steady-Luc Reporter Assay Kit
Reporter gene assays are an important tool for biomedical and pharmaceutical researchers to monitor cellular events associated with gene expression, gene ...
#19. Luciferase Reporter Assay Kit - APExBIO
Firefly luciferase is a sensitive reporter for gene regulation and function study. The Luciferase Reporter Assay Kit offers a fast and easy way of sensitive way ...
#20. Luciferase reporters—Ready-To-Glow secreted luciferase assay
Our Ready-To-Glow Secreted Luciferase Reporter Assay is a complete system designed for use in a transcription reporter system based on luciferase activity in ...
#21. Luciferase reporter assays to monitor interferon signaling ...
Luciferase-based reporter gene assays to measure gene expression have been used since the late 1980s for various purposes (de Wet et al., 1987; ...
#22. Application of the dual-luciferase reporter assay to the ...
The dual luciferase assay has been widely used in cell lines to determine rapidly and accurately the activity of a given promoter. Although this ...
#23. Automated Cell Transfection Reporter Gene Assay
Reporter gene assays are used to study gene expression and regulation. The most common applications include the testing of cis-regulatory sequences such as ...
#24. Luciferase Reporter Gene Assay, high sensitivity - Sigma-Aldrich
Luciferase Reporter Gene. Assay, high sensitivity y Version 20. Content version: March 2016. Chemiluminescent assay for the quantitative determination of ...
#25. Secreted Alkaline Phosphatase Reporter Assay Kit ...
Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric). Validated: Enzyme Activity. Backed by our 100% Guarantee.
#26. Luciferase Assay - CSH Protocols
In this protocol, cells transfected with a luciferase reporter plasmid are lysed using a detergent-containing buffer. Cell debris is removed by ...
#27. Reporter-Assays | Biomol GmbH - Life Science Shop
The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell ...
#28. SuperLight™ Luciferase Reporter Gene Assay Kit
Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.
#29. Luciferase Reporter Assay Kit (ab287865) - Abcam
Luciferase Reporter Assay Kit ab287865 (previously known as Luciferase Reporter Assay Kit K801) provides a simple means for detecting luciferase activity in ...
#30. Luciferase reporter assay - Bio-protocol
Luciferase Reporter Assay. 1. Plate cells of interest to 60-70% confluence in 3 wells of a 6 well-plate (biological triplicate).
#31. LightSwitch™ Luciferase Assay System - Active Motif
The LightSwitch™ Luciferase Assay System from Active Motif is a complete solution for gene regulation studies in mammalian cells, with over 18000 pre-cloned ...
#32. Dual Luciferase Reporter (DLR) assay certification
The Dual-Luciferase Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two step reaction that uses two ...
#33. Luciferase Reporter Assays: An Overview - G-Biosciences
Genetic reporters are widely used in drug development, biomedical research, and biochemistry. In a nutshell, the reporter gene is fused with ...
#34. Secreted reporter assay enables quantitative and longitudinal ...
Secreted Neuronal Activity Reporter (SNAR) is a dual luciferase live-cell assay. Expression of the immediate early gene Arc/Arg3.1 is rapidly ...
#35. Development of a Fluorescence-Based, High-Throughput ...
In this work, we report a new assay to identify inhibitors of 3CL pro. Our reporter is based on a green fluorescent protein (GFP)-derived ...
#36. Luciferase Assays | Cell Viability Assay Kits - Biotium
Most cells have no intrinsic bioluminescence or luciferase activity, making luciferase reporter assays highly specific and sensitive. The bioluminescent signal ...
#37. How to avoid misinterpretation of dual reporter gene assay ...
The activity of nuclear receptors (e.g., pregnane x receptor, PXR) can be assessed by luminescence-based dual reporter gene assays.
#38. reporter assay Archives - Promega Connections
In a typical genetic reporter assay, cells are transfected with a vector that contains the sequence of interest cloned upstream of a reporter gene, and the ...
#39. Secreted dual reporter assay with Gaussia luciferase ... - PLOS
As a case in point, we have developed a panel of Gaussia luciferase reporter genes for transcriptional activation assays with estrogen and ...
#40. Human Retinoic Acid Receptor Alpha Reporter Assay System
Bioanalysis and compound screening using Cayman or qualified commercial assay kits · Development and qualification of fit-for-purpose assays in a variety of ...
#41. Reporter gene assays and in vivo imaging - BioThema
The luc gene coding for firefly luciferase may be attached to another gene of interest. The expressing of this gene may then be estimated from the level of ...
#42. Dinucleotide tag‐based parallel reporter gene assay method ...
Therefore, a parallel reporter assay system capable of bias-free gene regulatory function investigation of risk SNPs remains lacking, regardless ...
#43. Principle of the reporter gene assay. A plasmid containing a ...
A plasmid containing a reporter gene downstream of the natural gene promoter is inserted into a recombinant cell, and activation of the promoter results in ...
#44. Characterization and Use of TurboLuc Luciferase as a ...
Luciferase-based reporter assays are powerful tools for monitoring gene expression in cells because of their ultrasensitive detection ...
#45. Systematic analysis of bicistronic reporter assay data
Abstract. Bicistronic reporter assay systems have become a mainstay of molecular biology. While the assays themselves encompass a broad range of diverse and ...
#46. Customized Functional Reporter Assay Service
Case 1. Response Element (RE) DNA Sequence Optimization. The response window is an important indicator for reporter gene assay cell line . Rich experience in RE ...
#47. Pre-validation of a reporter gene assay for oxidative stress for ...
For this we used an NRF2 specific chemically activated luciferase expression reporter gene assay derived from the human U2OS osteosarcoma ...
#48. Dual-reporter assay using two secreted luciferase genes
Luciferase is used as a reporter enzyme to estimate gene expression in prokaryotic or eukaryotic cells because the amount of luciferase ...
#49. Luciferase Assay - AbVideo™ - Support - Abnova
The Luciferase Assay is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Through the assay, light is produced by converting the ...
#50. Secreted Reporter Assay Enables Quantitative and ... - eNeuro
1 gene with a secreted reporter, Gaussia luciferase (Gluc), to quantify neuronal activity without sacrificing the neurons. The reporter ...
#51. A simple and robust reporter gene assay for measuring the ...
In this study, we developed a reporter gene assay (RGA) based on a RAW264.7 cell line stably expressing luciferase reporter under the control of nuclear factor- ...
#52. The Lowdown on Transcriptional Reporters - Tempo Bioscience
The reporter gene is transcribed and translated by the transfected cells, and depending on the actual reporter gene used, its activity may be ...
#53. G Protein Signaling: Methods and Protocols
In this chapter, a luciferase reporter gene assay system is described for the ... This assay system, in which ligand-stimulated production of the reporter ...
#54. The LightSwitch™ 3´UTR Reporter GoClone® Collection
... human 3′UTR reporter GoClone ® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for ...
#55. Genetic Analysis: Genes, Genomes, and Networks in Eukaryotes
In this hypothetical example, the reporter gene is expressed in the brain and the anterior central nervous system (CNS). Because the reporter gene comple- ...
#56. G Protein-Coupled Receptors, Part B - 第 24 頁 - Google 圖書結果
Thus, the STAT3-driven luciferase reporter assay can be useful for monitoring both G protein-dependent and -independent activity of any GPCR upon ligand ...
#57. Handbook of Assay Development in Drug Discovery
protocol, 250 results, 250 PerkinElmer - LANCE cAMP assay, 252–254 kit components, ... 392–393 example LDH-release assay data, 394 reporter gene assay data ...
#58. Plant Molecular Biology Manual - Google 圖書結果
Selection and screening for T-DNA-induced reporter gene fusions Analysis of the frequency of T-DNA-induced reporter gene fusions indicated that T-DNA ...
#59. DNA-Protein Interactions: Methods and Protocols
Current protocols for luciferase assays also include the mea- surement of a second reporter gene as an internal control. This particular procedure is known ...
#60. Live Cell Reporter Assay 분비형 Reporter (Metridia Luciferase)
Ready-To-Glow Secreted Luciferase Reporter Assay ... Clontech의 live cell secreted reporter는 다른 transcriptional reporter 이상으로 많은 장점을 갖고 있다.
#61. MicroRNA Signaling - 第 56 頁 - Google 圖書結果
... using the Dual-Luciferase Reporter Assay System (Promega, United States). ... ChIP assays were performed using an EZ-Magna ChIPTM HiSens kit (Millipore, ...
#62. ATCC: The Global Bioresource Center | ATCC
Learn how our new checkpoint luciferase reporter cells can deliver high performance and reproducibility to your screening assays. Read the blog ...
#63. Predictive, Prognostic Biomarkers and Therapeutic Targets in ...
Luciferase reporter assay was conducted to evaluate the miR205-5p binding site within the 3' untranslated region (UTR) of circ_NOTCH3.
#64. MicroRNA Let-7i regulates innate TLR4 pathways in AS | IJGM
Bioinformatics and Luciferase Reporter Assay. Target genes for miRNAs were predicted using TargetScan web tools, as previously described. To ...
#65. Identification of Botanicals that Unmask β-Glucan from the Cell ...
This screen measures the activity of a reporter gene in response to ... This screen, together with direct killing/growth inhibition assays, ...
#66. SHED-derived exosomes ameliorate hyposalivation caused ...
Double luciferase reporter gene assay. A rat ZO-1 promoter fragment was amplified by PCR and inserted into the pGL3 vector. HEK293T cells were ...
#67. Whole-Transcriptome Sequencing of Antler Tissue Reveals ...
(O,P) Dual luciferase reporter gene assay to demonstrate the binding site of miR-4286-R+1 to circRNA2829 and FOXO4; (Q) RT-qPCR to detect the expression levels ...
#68. Thermo Fisher Launching Broad PCR Test Menu Through ...
The company will launch 37 CE-IVD-marked assays this year, mostly for infectious diseases, for use on the QuantStudio 5 Dx ... Apr 18, 2023 | staff reporter.
#69. 515-5p and Up-regulating CXCL6 - Bentham Science Publisher
... was confirmed through bioinformatics, RNA immunoprecipitation (RIP) assay, dual-luciferase reporter gene assay and RNA pull-down assay.
#70. Vector Core - Center for Systems Biomedicine - UCLA Health
Titer determination of retroviral vectors depends on whether the transfer vector co-expresses a readily detectable reporter gene (e.g. GFP) or a selectable ...
#71. Small-Molecule Polθ Inhibitors Provide Safe and Effective ...
DNA polymerase theta (Polθ, encoded by the POLQ gene) is a DNA repair ... Dual-Luciferase Reporter Assay Kit (Promega) and luminescence was ...
#72. Integrated DNA Technologies IDT
Prove it. We'll help. Our custom oligo synthesis platforms provide innovative research tools for genomics applications using NGS, CRISPR, qPCR, ...
#73. Enrichr - Ma'ayan Laboratory - Computational Systems Biology
Input data. Expand a gene, a term, or a variant into a gene set: Try ...
#74. Ghana approves Oxford's malaria vaccine for children aged 5 ...
Screening of SARS-CoV-2 antivirals through a cell-based RNA-dependent RNA polymerase (RdRp) reporter assay. Timsy Uppal et al., Cell Insight ...
#75. Research Associate Jobs in USA, CA, Foster City
Troubleshoot and optimize assays as needed, identifying and applying novel ... reporter-based assays, ELISA, flow cytometry, western blot, CRISPR KO);
#76. Zebrafish fluorescence microscopy imaging
Automate your zebrafish screening assay with the Imaging Machine ! ... Imaging oscillating reporter gene expression to study pattern formation in zebrafish ...
#77. Cellranger Source Code. Cell Ranger is a set of analysis ...
... velocity analysis on 10x Genomics Single Cell Gene Expression data. ... including the following: (1) cell reporter assay using artificial APCs,,,, ...
#78. Postdoctoral Scientist - Melmed Lab - Department of Medicine
Luciferase reporter gene assay; Site-directed mutagenesis; Glutathione S-transferase (GST) pull-down experiments
#79. US reporter held by Russia on spying charges to stay in jail
A Russian judge has ruled that American journalist Evan Gershkovich must remain behind bars on espionage charges.
#80. New technique could help diagnose ... - Yahoo News UK
Nilima Marshall, PA Science Reporter ... known as alpha-synuclein seed amplification assay (alphaSyn-SAA), can accurately identify people ...
#81. High Throughput Screening Market Insights, Forecast to 2030
Clinical applications (Reporter assay in biological systems analysis); In-vitro studies (Genetic reporter assays in cloning tools for ...
#82. Biology Genetic Engineering Answers - Palato
progress of gene therapy and other medical marvels--yet Grace argues ... of bacterial reporter assays. A variety of assay formats will be illustrated, and.
#83. MedChemExpress: Master of Bioactive Molecules | Inhibitors ...
Amino Acid Derivatives · Biochemical Assay Reagents · Fluorescent Dye · Isotope-Labeled ... Cell Proliferation and Cytotoxicity · Reporter Gene Testing ...
#84. ReadyGo Diagnostics, Gemina Laboratories Sign ... - 360Dx
Apr 19, 2023 | staff reporter. Save for later ... The firms will develop a 30-minute assay to be used on the near-patient Geo instrument.
#85. New technique could help diagnose Parkinson's disease ...
Saffron Walden Reporter ... known as alpha-synuclein seed amplification assay (alphaSyn-SAA), can accurately identify people who are at risk ...
#86. Luciferase 060606
The Luciferase Assay System incorporates coenzyme A (CoA) for improved kinetics, ... A commonly used reporter gene is the luciferase gene from the firefly ...
#87. Home page for MMWR
The Morbidity and Mortality Weekly Report (MMWR) Series is prepared by the Centers for Disease Control and Prevention (CDC).
#88. Game-Changing New Parkinson's Test Paves Way ... - Forbes
The technique, known as the alpha-synuclein seed amplification assay, ... I am a senior reporter for the Forbes breaking news team, ...
#89. New technique could help diagnose Parkinson's disease ...
Dumbarton and Vale of Leven Reporter ... known as alpha-synuclein seed amplification assay (alphaSyn-SAA), can accurately identify people ...
#90. RNA Modification in Human Cancers: Roles and Therapeutic ...
The DAVID online tool was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and ... A Dual Luciferase Reporter Assay System (Promega, WI, ...
#91. Parkinson's disease breakthrough reported - CTV News
The technique is called a-synuclein seeding amplification assay, and it can detect an abnormal protein linked with Parkinson's disease in ...
#92. How To Use Flow Bindings. They have one large strap section ...
Luciferase reporter assay and RNA Binding Protein Immunoprecipitation (RIP) were used to ... Flow cytometry–based internalization assay using cell surface ...
#93. MedWatch: The FDA Safety Information and Adverse Event ...
Prescription and over-the-counter medicines · Biologics such as blood components, blood/plasma derivatives and gene therapies. · Medical devices such as hearing ...
reporter assay 在 Introduction to Reporter Gene Assays - YouTube 的推薦與評價
Learn more about the basic concept of reporter assays using luciferase, and how a dual- luciferase assay works. To learn more about this ... ... <看更多>